A Fine-Scale Phylogenetic Analysis of Free-Living Burkholderia Species in Sugarcane Field Soil
نویسندگان
چکیده
The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10(7) g(-1) soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.
منابع مشابه
Short Communication A Fine-Scale Phylogenetic Analysis of Free-Living Burkholderia Species in Sugarcane Field Soil
1Environmental Biofunction Division, National Institute for Agro–Environmental Sciences, 3–1–3 Kannondai, Tsukuba, Ibaraki 305–8604, Japan; 2Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido, 2–17–2–1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062–8517, Japan; 3Research Institute for Environmental Management Technology, AIS...
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عنوان ژورنال:
دوره 29 شماره
صفحات -
تاریخ انتشار 2014